Assessment of Novel C-4 Methylated Tetraketide by HPLC-SPE-TT in Saccharicola sp., an Endophytic Fungus in Eugenia jambolana Lam.

A new compound named methyl (6S,7S,2E,4E)-6,7-dihydroxy-4,6-dimethyl octanoate was isolated from Saccharicola sp. using high performance liquid chromatography-solid phase extraction-transfer tube (HPLC-SPE-TT), together with four known compounds, fusaric acid, trans-4-hydroxymellein, thymidine and adenosine. Saccharicola sp. was isolated from the stems of Eugenia jambolana Lam. (Myrtaceae) as endophytic fungus. The structure of this novel substance was established based on its spectroscopic data, including 1H and 13C nuclear magnetic resonance (NMR), 2D NMR techniques and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS). This paper deals with the first report of its kind on this compound in Saccharicola sp.

本研究采用高效液相色谱-固相萃取-转移管联用法（high performance liquid chromatography-solid phase extraction-transfer tube, HPLC-SPE-TT），从一株分离自乌墨（Eugenia jambolana Lam.，桃金娘科Myrtaceae）茎部的内生真菌萨卡霉菌属（Saccharicola sp.）中，分离得到一种命名为(6S,7S,2E,4E)-6,7-二羟基-4,6-二甲基辛酸甲酯的新型化合物，同时还分离得到四种已知化合物：镰孢酸（fusaric acid）、反式-4-羟基蜜菌素（trans-4-hydroxymellein）、胸苷（thymidine）及腺苷（adenosine）。该新型化合物的结构通过其波谱数据得以确证，包括氢核磁共振波谱（¹H NMR）、碳核磁共振波谱（¹³C NMR）、二维核磁共振波谱（2D NMR）技术以及高分辨电喷雾电离质谱（high-resolution electrospray ionization mass spectrometry, HR-ESI-MS）。本研究首次在萨卡霉菌属（Saccharicola sp.）中报道该化合物。