LEPIDOPTERAN-ACTIVE CRY1DA1 AMINO ACID SEQUENCE VARIANT PROTEINS

Engineered CrylDa amino acid sequences are provided that exhibit unproved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring CrylDa protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins， as well as recombinant plants， plant parts， and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

本研究提供了经过工程改造的Cry1Da（CrylDa）氨基酸序列，与天然存在的Cry1Da蛋白毒素相比，此类序列展现出改良的鳞翅目昆虫杀虫活性，且鳞翅目杀虫谱得到拓展。同时提供了可用于在植物中表达上述改良蛋白的多核苷酸序列。特定实施例提供了含有杀虫抑制剂量的工程改造蛋白的组合物，以及携带有编码一种或多种上述改良工程蛋白的多核苷酸构建体的重组植物、植物组织及种子。