Standard area diagram set for scab quantification in trilobed leaves of the sour passion fruit

ABSTRACT: The study aimed to develop and validate a standard area diagram set (SADs) to improve accuracy, precision, and reproducibility in the assessment of scab severity in trilobed leaves of the sour passion fruit. The SADs comprised six severity levels (1%; 3%; 6%; 11%; 21%; and 33%). The SADs increased the accuracy of the estimates by reducing the constant and systematic errors, and the tendency to overestimate the actual severity, limited the number of evaluators who presented this trend, in addition to increasing the bias correction factor. The inexperienced evaluators were the ones that benefited the most from the SADs use. Once the SADs was introduced, 80% and 100% evaluators gave estimates free from constant and systematic errors, respectively. The improvement in precision was verified by the increase in the coefficient of determination and Lin’s concordance correlation coefficient, by reducing absolute errors and increasing the reproducibility of the estimates.

摘要：本研究旨在开发并验证一套标准面积图集（standard area diagram set，SADs），以提升酸百香果三裂叶疮痂病病情严重程度评估的准确性、精密度与可重复性。该SADs包含6个病情严重程度等级（1%、3%、6%、11%、21%及33%）。该标准面积图集通过降低恒定系统误差与高估实际病情严重程度的倾向，提升了评估准确率，同时限制了出现此类高估倾向的评估者数量，此外还提高了偏差校正因子。经验不足的评估者是使用该SADs后获益最大的群体。在引入该SADs后，80%与100%的评估者所给出的评估结果分别不存在恒定系统误差与系统误差。本研究通过判定系数与林氏一致性相关系数的提升、绝对误差的降低以及评估结果可重复性的增强，验证了评估精密度的改善。