Additional file 1 of Anaerobic derivates of mitochondria and peroxisomes in the free-living amoeba Pelomyxa schiedti revealed by single-cell genomics

Additional file 1: Table S1. Statistics of Pelomyxa schiedti assemblies were compared with those of Mastigamoeba balamuthi. Table S2. Proteins targeted to MRO of Pelomyxa schiedti. Localization of proteins was predicted by several tools, as listed in columns E - J. Mitochondrial predictions are highlighted by white text on blue background. Column K shows final inferred prediction of localization. Abbreviations: cyt, cytosolic; ER, endoplasmic reticulum; extracell, extracellular; mit, mitochondrial; nuc, nuclear; Other, other localization; perox, peroxisomal; PM, plasma membrane; sec, secretory system; SP, signal peptide; -, protein not localized in MRO; +, protein localized in MRO; +?, protein localized in MRO with low confidence. Table S3. Proteins required for peroxisome biogenesis and targeted to peroxisome. Proteins identified in Mastigamoeba balamuthi are highlighted by white text on blue background. Proteins were considered peroxisome-targeted, if they contained PTS1 (SRI or [SAP][KR][LM]) or PTS2 motif (R[LIV](x5)HL), and/or were predicted by PTS1 predictor [83]. Table S4. Primers used in this study.

附加文件1：表S1。施氏泡囊变形虫（Pelomyxa schiedti）的基因组组装统计结果与巴拉姆希鞭毛变形虫（Mastigamoeba balamuthi）进行了对比。
表S2。定位于施氏泡囊变形虫线粒体相关细胞器（mitochondrion-related organelle, MRO）的蛋白质。蛋白质的亚细胞定位通过多种工具进行预测，预测结果列于E至J列。线粒体定位预测结果以蓝底白字标注。K列展示最终推断得到的蛋白质定位预测结果。缩写说明：cyt（胞质，cytosolic）；ER（内质网，endoplasmic reticulum）；extracell（细胞外，extracellular）；mit（线粒体，mitochondrial）；nuc（细胞核，nuclear）；Other（其他定位）；perox（过氧化物酶体，peroxisomal）；PM（质膜，plasma membrane）；sec（分泌系统，secretory system）；SP（信号肽，signal peptide）；-：表示蛋白质未定位于MRO；+：表示蛋白质定位于MRO；+?：表示蛋白质低置信度定位于MRO。
表S3。参与过氧化物酶体生物发生且定位于过氧化物酶体的蛋白质。在巴拉姆希鞭毛变形虫中鉴定到的蛋白质以蓝底白字标注。若蛋白质含有PTS1基序（SRI或[SAP][KR][LM]）或PTS2基序（R[LIV](x5)HL），且/或通过PTS1预测工具[83]得到预测结果，则认定其为过氧化物酶体靶向蛋白质。
表S4。本研究使用的引物。