GSTE2 allelic variants from the An. gambiae Kisumu and ZAN/U strain used for recombinant protein expression.
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The position of variant amino acids proximal to the putative DDT binding site are shown; together with the specific activity of the recombinant GSTE2 with substrate CDNB. Protein concentrations were determined using a commercial assay (Fluka – Sigma-Aldrich) based on Bradford assay chemistry[40]. CDNB activity was determined by colorimetric assay and spectrophotometric reading.
创建时间：
2015-12-02
