Additional file 1 of Sputum bacterial load and bacterial composition correlate with lung function and are altered by long-term azithromycin treatment in children with HIV-associated chronic lung disease

Additional file 1: Figure S1. A bar plot of the taxa and their relative abundance of the extraction and sequencing mock controls compared to manufacturer profiles. Figure S2. A scatterplot showing the correlation between samples repeated within a run (WR, n = 74) and between runs (BR, n=28). Figure S3. A scatterplot showing the spread of biological samples (n=960) and the negative controls (primestore, n=43) 16S copies vs final number of reads (A1 and A2), Shannon alpha diversity index (B1 and B2) and age of participant in years (C1 and C2). Figure S4. Ordination plots of showing the spread of biological samples (n=960) and the negative controls (primestore, n=43) coloured by their 16S copies. Figure S5. Ordination plots showing the spread of biological samples (n=960) and the negative controls (primestore, n=43) coloured by their number of reads. Figure S6. Ordination plots showing the spread of biological samples (n=960) and the negative controls (primestore, n=43) coloured by the age of the participant. Figure S7. Rarefaction curves showing number of ASVs detected and 16S copies of samples. Figure S8. Rarefaction curves showing number of ASVs detected and number of reads of samples. Figure S9. Bar plot showing the profiles of biological samples with 100 to

附加文件1：
图S1：展示提取及测序用模拟对照（mock control）的类群及其相对丰度，并与厂商给定的特征谱进行对比的柱状图。
图S2：散点图，展示单批次内重复样本（WR，n=74）与批次间重复样本（BR，n=28）之间的相关性。
图S3：散点图，展示960份生物样本与43份primestore阴性对照（primestore）的16S拷贝数与最终测序读段数（A1、A2）、香农α多样性指数（Shannon alpha diversity index）、受试者年龄（单位：年）的分布关系。
图S4：排序图，展示960份生物样本与43份primestore阴性对照的分布情况，并以其16S拷贝数进行着色。
图S5：排序图，展示960份生物样本与43份primestore阴性对照的分布情况，并以其测序读段数进行着色。
图S6：排序图，展示960份生物样本与43份primestore阴性对照的分布情况，并以受试者年龄进行着色。
图S7：稀释曲线（rarefaction curves），展示样本中检测到的扩增子序列变体（Amplicon Sequence Variant，ASV）数量与16S拷贝数。
图S8：稀释曲线，展示样本中检测到的扩增子序列变体数量与测序读段数。
图S9：柱状图，展示100至的生物样本特征谱。