FAIRsharing record for: Minimum Information Required for A Glycomics Experiment - Mass Spectrometric Analysis

This FAIRsharing record describes: MIRAGE (Minimum Information Required for A Glycomics Experiment) was created to improve the quality of glycomics data in the scientific literature. Researchers seeking to understand the biochemical structure–function relationships of carbohydrates require detailed descriptions of the assay conditions and the experimental results. Currently, these data are insufficiently reported in the literature. A basic description on the sample preparation workflow is required. In contrast to proteomics, different types of glycoconjugates require partially different release approaches, which in turn can have direct influence on the following conditions/parameters: released glycans or still attached to protein/lipid; type of glycan (N-glycan, O-glycan, proteoglycan fragment); and sample pre-treatment prior MS-Analyses (non, reduced, permethylated, endo/exoglycosidase digested, fluorescent label, online/offline LC-separation).

本FAIRsharing记录描述如下：MIRAGE（Minimum Information Required for A Glycomics Experiment，即糖组学实验最低信息规范）旨在提升学术文献中糖组学数据的质量。致力于解析碳水化合物生化结构与功能关联的研究人员，需要获取实验条件及实验结果的详细描述，但当前学术文献中此类数据的报告充分性仍有待提升，因此需对样品制备流程提供基础说明。与蛋白质组学（proteomics）不同，不同类型的糖结合物（glycoconjugates）需要采用存在差异的解离方法，而该方法又会直接影响后续的实验条件与参数，包括解离后的聚糖（glycan）或仍结合于蛋白质/脂质的聚糖、聚糖类型（N-聚糖（N-glycan）、O-聚糖（O-glycan）、蛋白聚糖片段（proteoglycan fragment））以及质谱分析（MS-Analyses）前的样品预处理方式：未处理、还原、全甲基化、内切/外切糖苷酶消化、荧光标记、在线/离线液相色谱（LC）分离。