Dataset of live-cell movies of single PolC-PAmCherry molecules in Bacillus subtilis cells with high and low fluorescent backgrounds.

This is the experimental data referenced in our manuscript entitled “SMALL-LABS: An algorithm for measuring single molecule intensity and position in the presence of obscuring backgrounds .” These live-cell single-molecule imaging movies were used as a test of the SMALL-LABS single-molecule image analysis algorithm.

The dataset comprises two movies; each one is provided both as a .tif stack and as an .avi file. The movie called “low_bg” has a standard low background, and the movie called “high_bg” includes a high fluorescent background produced by an external 488-nm laser.

本数据集为本课题组题为《SMALL-LABS：一种可在干扰背景下检测单分子强度与位置的算法》的学术手稿中所引用的实验数据。本次测试采用的活细胞单分子成像序列，用于对SMALL-LABS单分子图像分析算法开展验证测试。
本数据集包含两部成像序列，每部均同时提供.tif堆叠（.tif stack）与.avi文件两种格式。其中命名为“low_bg”的序列为标准低背景成像数据，命名为“high_bg”的序列则包含由外置488nm激光器产生的强荧光背景。