(A) Localization of the cargo prApe1 in the MKO () strain, and localization of the receptor Atg19 and the adaptor Atg11 in the MKO () strain

The MKO () strain and the MKO () strain transformed with a plasmid expressing YFP-Atg19 (pYFPATG19(416)) or HA-tagged CFP-Atg11 (pCuHACFPATG11(414)) were grown in selective SMD medium to mid-log phase and observed by fluorescence microscopy. DIC, differential interference contrast. (B) Colocalization of prApe1, Atg19, and Atg11. The MKO () strain was transformed with a plasmid expressing YFP-Atg19, HA-tagged CFP-Atg11, or both, grown to mid-log phase and observed by fluorescence microscopy. When Atg19 was coexpressed with prApe1 in the MKO () strain, the cargo prApe1 colocalized with the receptor Atg19 (top). When Atg19 was absent, the adaptor Atg11 (arrows) did not colocalize with the cargo (middle). When prApe1, Atg19, and Atg11 were all present, the three proteins colocalized to the same structure (bottom). Bars, 2.5 μm.<b>Copyright information:</b>Taken from "In vivo reconstitution of autophagy in "The Journal of Cell Biology 2008;182(4):703-713.Published online 25 Aug 2008PMCID:PMC2518709.