Supporting data for thesis titled "Are calcium- and phosphate-containing fluoride varnishes more effective than the conventional fluoride varnish in preventing early childhood caries?"

This data contains supporting resources for the thesis titled "Are calcium- and phosphate-containing fluoride varnishes more effective than the conventional fluoride varnish in preventing early childhood caries?" The thesis is pivoted on a randomised clinical trial that started to recruit children in 2019. The baseline characteristics of the study participants is published in International Dental Journal (Article title: Risk indicators for non-cavitated and cavitated carious lesions in preschool children- Manchanda et al., 2023). The follow-up data of this two-year randomised trial is under consideration for publication in a refereed journal at the time of publishing this data. The first dataset associated with these publications was generated utilizing the demographic characteristics of the study population, knowledge attitude and practices regarding oral health, and clinical parameters (including visile plaque index and dental caries) at the level of teeth. A subset of the above population was randomly sampled from the above dataset and saliva and plaque were collected to evaluate the quantity of S.mutans and L. fermentum using the qRT-PCR technique. The baseline characteristics of that subset with the microbiological counts in saliva and plaque samples are published (Article title: Manchanda S, Cheung BPK, Lee GHM, Lo ECM, Yiu CKY. Quantitative analysis of salivary and biofilm bacteria associated with cavitated and non-cavitated carious lesions in pre-school children. Arch Oral Biol. 2023;146:105607. doi: 10.1016/j.archoralbio.2022.105607.). At the time of this data publishing, the two-year follow-up of this subset is under peer review regarding the effect of three study varnishes in altering the microbiological counts.

本数据集为题为《含钙与磷酸盐的含氟涂料（fluoride varnish）相较传统含氟涂料，在预防儿童早期龋病方面是否更具疗效？》的学位论文提供配套支撑资源。该论文依托一项2019年启动儿童招募的随机临床试验展开。研究参与者的基线特征已发表于《国际牙科杂志》（论文标题：《学龄前儿童非龋洞与龋洞龋损的风险指标——Manchanda等，2023》）。在本数据集发布之际，该项为期两年的随机临床试验的随访数据正处于同行评审期刊的待刊审核阶段。与上述两篇发表文献相关的首个数据集，由研究人群的人口统计学特征、口腔健康知识-态度-行为情况，以及以牙为单位的临床指标（包括可视菌斑指数与龋病状况）生成。研究人群中的一个随机亚组从上述数据集中被抽取，采集其唾液与菌斑样本，通过定量逆转录聚合酶链反应（qRT-PCR）技术，检测变形链球菌（S.mutans）与发酵乳杆菌（L. fermentum）的载量。该亚组的基线特征及唾液、菌斑样本中的微生物计数已发表（论文信息：Manchanda S, Cheung BPK, Lee GHM, Lo ECM, Yiu CKY. 《学龄前儿童龋洞与非龋洞龋损相关的唾液与生物膜细菌定量分析》，《口腔生物学档案》，2023年；146卷：105607，DOI: 10.1016/j.archoralbio.2022.105607）。在本数据集发布之时，该亚组的两年随访数据正处于同行评审阶段，评审内容为三种研究用含氟涂料对微生物载量的影响。