Data for: A new reverse transcription-insulated isothermal polymerase chain reaction (RT-iiPCR) assay for DHAV-3 detection based on the POCKIT™ system

These documents contain some important dates about the development and evaluation of this RT-iiPCR of DHAV-3. And we listed as follows: Fig 1 The linear relation of every dilution detected by rRT-PCR. Table 1 Reference strains and isolates used in this study for specificity testing of RT-iiPCR. Table 2 Primer information rRT-PCR assay. Table 3 Evaluation of the sensitivity of the rRT-PCR and RT-iiPCR assays to DHAV-3. Table 4 Performance evaluations of the PetNADTM and TRizol RNA extraction methods for the detection of DHAV-3 by rRT-PCR and RT-iiPCR, respectively. Table 5 The Contingency table for analysis of the level of agreement between the rRT-PCR and the RT-iiPCR assays for the detection of DHAV-3 in liver samples.

本数据集包含与鸭甲型肝炎病毒3型（DHAV-3）的RT-iiPCR方法开发及评估相关的重要数据，具体列表如下：图1 实时逆转录聚合酶链反应（rRT-PCR）对各稀释度样本的检测线性关系；表1 本研究中用于RT-iiPCR特异性检测的参考毒株及分离株；表2 rRT-PCR检测方法的引物信息；表3 rRT-PCR与RT-iiPCR检测方法对DHAV-3的敏感性评估；表4 分别采用rRT-PCR与RT-iiPCR检测DHAV-3时，PetNADTM与Trizol两种RNA提取方法的性能评估；表5 用于分析rRT-PCR与RT-iiPCR检测肝脏样本中DHAV-3时结果一致性水平的列联表。