MOESM5 of Enhancing antibody folding and secretion by tailoring the Saccharomyces cerevisiae endoplasmic reticulum

Additional file 5. Result from the frequency analysis showing the strains in the 95th and 5th percentile of individual measurements of specific product yield in each temperature and strain background. To determine which strains were enriched among the highest and lowest values of specific product yield, we conducted a frequency analysis for each strain background, separately for each temperature. Frequency represents the absolute count of measurements from the strain inside the percentile and the relative frequency is the frequency normalized to the total count of measurements in the percentile. P values were calculated with Wilcoxon signed rank test using the respective background strain measurements in the same temperature as the reference.

附加文件5。本文件为频率分析所得结果，展示了在各温度与菌株背景（strain background）条件下，单次特定产品得率测量值分别处于第95百分位与第5百分位的菌株。为明确在特定产品得率的最高与最低取值区间内存在富集现象的菌株，我们针对每一类菌株背景分别开展频率分析，且所有分析均按不同温度分组进行。此处的频率指对应百分位区间内某菌株的测量值绝对计数；相对频率则是将该计数归一化至该百分位区间内总测量值数后的结果。P值通过威尔科克森符号秩检验（Wilcoxon signed rank test）计算得到，检验时以相同温度下对应背景菌株的测量值作为参照。