Influence of sodium butyrate administered in ovo on gene expression in the brain of broiler chickens

The following research assessed the effect of sodium butyrate (SB) on the expression of genes related to innate immune response and small heat shock proteins in the brains of broiler chickens. For this purpose 1000 eggs were incubated and divided into 4 groups. Three were injected with either 0.1, 0.3, or 0.5% doses of SB and the last one only with saline. Samples were collected post-mortem on day 42 of rearing. RNA isolated from the brain was tested for gene expression using a panel of the following: HSPB1, HSPB5, HSPB8, HSPB9, HSP70, IL-1β, IL-2, IL-4, IL-6, IL-8, IFNγ, IFNβ, TLR4, GR, BDNF, GSK3β, NR2A, CRH, NF-κB p65.The dataset presents raw data related to the publication: Bełdowska, A., & Dunisławska, A. (2024). Influence of sodium butyrate administered in ovo on gene expression in the brain of broiler chickens. Animal Science Papers & Reports, 42(4). https://doi.org/10.2478/aspr-2023-0048The data was obtained as a part of a project entitled "ActEpi: Activation of the epigenetic mechanisms in poultry through a programming of the gut microbiota", No. UMO-2021/43/D/NZ9/01548, financed by the National Science Center

本研究旨在评估丁酸钠（sodium butyrate, SB）对肉鸡大脑内天然免疫应答相关基因及小型热休克蛋白（small heat shock proteins）表达的影响。实验共孵化1000枚鸡蛋并随机分为4组，其中3组分别注射0.1%、0.3%或0.5%剂量的丁酸钠，剩余1组仅注射生理盐水作为对照。饲养至42日龄时剖杀采集样本，从脑组织中分离核糖核酸（RNA），采用包含以下基因的检测组合开展基因表达分析：HSPB1、HSPB5、HSPB8、HSPB9、HSP70、IL-1β、IL-2、IL-4、IL-6、IL-8、IFNγ、IFNβ、TLR4、GR、BDNF、GSK3β、NR2A、CRH、NF-κB p65。本数据集包含与该学术论文配套的原始数据：Bełdowska, A. 与 Dunisławska, A. (2024) 发表的《卵内施用丁酸钠对肉鸡大脑基因表达的影响》，刊载于《Animal Science Papers & Reports》第42卷第4期，DOI: 10.2478/aspr-2023-0048。本数据源自题为"ActEpi：通过肠道菌群编程激活家禽表观遗传机制"的研究项目，项目编号为UMO-2021/43/D/NZ9/01548，由波兰国家科学中心资助。