Additional file 7 of An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

Additional file 7. Synthetic DNA fragment map, sequence, translation and features, coding for optimized SP-PRO-NHSSP gene with deleted C-terminal 4 aa and His6-tag. Secretion peptide native—marked in green, propeptide—marked in yellow.

补充材料7。本材料包含合成DNA片段的图谱、序列、翻译产物及特征信息，该片段编码经优化的SP-PRO-NHSSP基因，该基因缺失C端4个氨基酸（amino acids，aa）并带有His6标签（His6-tag）。其中天然分泌肽以绿色标注，前肽以黄色标注。