Expression data from PDGF-B induced murine gliomas at different progression stages

The different phases of tumor immunoediting in vivo were dissected thanks to a murine model of glioma induced by PDGF-B overexpression. We show that low-grade gliomas are highly immunostimulatory and that the adaptive immune system prevents the development of secondary tumor in syngeneic mice. During tumor progression, glioma cells downregulate immunostimulatory genes and the immune infiltrate becomes pro-tumorigenic. Cells from early-onset / low grade and late-onset / high grade were explanted, dissociated and sorted by FACS, basing on the expression of the EGFP transgene expressed together with PDGF-B as a bicistronic transcript. RNA extraction, sample labeling, array hybridization and scanning were carried out by Genopolis Consortium for Functional Genomics - (University of Milano-Bicocca, Italy , Dept. of Biotechnology and Bioscience)