Thomas Maresca, Edward Salmon (2011) CIL:25307, Drosophila melanogaster. CIL. Dataset

Movie showing kinetochore alignment during mitosis in Drosophila S2 cells expressing GFP-Ndc80 to label kinetochores. One pair of chromatids becomes detached from the spindle, and anaphase is delayed until the pair is recaptured.
Images were recorded at 60 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Maresca and Salmon, 2009 J Cell Biol 184:373-281.

本数据集展示果蝇S2细胞在分裂过程中表达GFP-Ndc80标记的着丝粒的动态对齐过程。一对染色单体从纺锤体上脱落，直至该对染色单体被重新捕获，有丝分裂的中期延迟。图像以60秒的间隔，使用尼康TE-200支架上的旋转盘共聚焦显微镜，配备100倍1.4NA计划阿贝正畸差分干涉（DIC）物镜拍摄，并由Hamamatsu Orca-ER CCD记录。详见Maresca和Salmon，2009年《细胞生物学杂志》第184卷第373-281页。