Excreted end-products of glucose and threonine metabolism by T. brucei long-slender bloodstream form (BSF) and procyclic form (PF) cell lines.

The extracellular PBS medium of trypanosomes incubated with 4 mM [U-13C]-glucose and 4 mM threonine was analyzed by 1H-NMR spectrometry to detect and quantify excreted end-products.a.i: RNAi cell lines tetracycline-induced for 5 to 10 days depending on the cell line and the experiments; .ni: uninduced RNAi cell lines.bPF and BSF were incubated at the 2×107 cells/mL (0.1 mg of proteins/mL) and 107 cells/mL (0.1 mg of proteins/mL), respectively.cNumber of experiments.dMean ± SD of 3 or 9 experiments of cells incubated in PBS containing 4 mM [U-13C]-glucose and 4 mM threonine (nmol/h/108 cells) are presented.eValues into brackets represent percent of each excreted molecules from [U-13C]-glucose metabolism.fRatio between glucose-derived and threonine-derived [13C]-acetate excreted.gNot detectable.