Genomic profiles of asbestos-induced mesothelioma developed in wild-type and BRCA1+/- SD rats

Intraperitoneal administration of crocidolite or chrysotile is an established method to induce peritoneal malignant mesothelioma(MM), which shares common genetic alterations to those in humans. We conducted high-resolution microarray comparative genomic hybridization mesothelioma tissues obtained from both wild-type and BRCA1+/- rats to evaluate the involvement of BRCA1 haploinsufficiency in carcinogenesis. The carcinogenesis protocol was started with 37 BRCA1+/- and 37 wild-type Jcl: SD rats. 26/37 (70.2%) of BRCA1+/- rats and 27/37 (72.9%) wild-type rats developed MMs in total. Genomes of 8 samples from MMs for each genotype (16 in total) were profiled on Agilent 180K rat genome CGH microarrays.