P7 Kdr-KO scRNA seq data

Lung tissue from four experimental groups Kdr^flox/floxand Tbx2^icreKdr^flox/floxunder maintained in normoxia or exposed to chronic hyperoxia were euthanized at P7. Lung tissue was dissected and snap-frozen in liquid nitrogen. Nuclei were prepared from frozen tissue using a nuclear isolation kit from 10X Genomics (10x Genomics, PN-1000494). For 10X Chromium, 10,000 nuclei were loaded per lane. The libraries were prepared according to manufacturer protocol (10X Genomics) using 3’V3 Kits (10X Genomics, PN-1000269) and were submitted for sequencing via a NovaSeq 6000 flow cell to a depth of 50,000 reads/cell. Raw sequencing data were processed using CellRanger pipeline

本研究取四个实验组的肺组织，涵盖两个基因型（Kdr^flox/flox与Tbx2^icreKdr^flox/flox），每个基因型分别设置常氧维持与慢性高氧暴露两个处理组，于出生后第7天（P7）对实验动物实施安乐死。解剖分离肺组织后，将其置于液氮中快速速冻。使用10X Genomics公司的细胞核分离试剂盒（货号：10x Genomics, PN-1000494）从冷冻组织中提取细胞核。针对10X Chromium单细胞测序平台，每个泳道上样10,000个细胞核。按照10X Genomics官方操作规程，使用3’V3试剂盒（货号：10X Genomics, PN-1000269）完成文库构建，随后通过NovaSeq 6000测序流通池进行测序，设定测序深度为50,000条读取序列/细胞。原始测序数据通过CellRanger分析流程进行处理。