Data and code from: Tetraspanin CD82 shapes EGFR signaling outcomes through nanoscale receptor organization

Tetraspanins are integral membrane proteins that play a crucial role in organizing and regulating cellular signaling by serving as scaffolds that compartmentalize receptors and other signaling molecules within membrane microdomains. Here, we report how the tetraspanin CD82 modulates the molecular organization and signaling of the Epidermal Growth Factor Receptor (EGFR), a key molecule involved in cellular proliferation, differentiation, and survival. Combining multi-color super-resolution microscopy with advanced image reconstruction and analysis techniques, we demonstrate that CD82 selectively associates with EGFR, promotes receptor oligomerization, and acts as a regulator of ligand-independent receptor phosphorylation in a palmitoylation-dependent manner. Additionally, CD82 promotes a more compact molecular organization of EGFR, which correlates with altered endocytosis and downstream signaling outcomes. These findings underscore the importance of tetraspanins in the spatial and funct..., , # Data and code from: Tetraspanin CD82 shapes EGFR signaling outcomes through nanoscale receptor organization

Dataset DOI: [10.5061/dryad.3n5tb2rz2](https://doi.org/10.5061/dryad.3n5tb2rz2)

## Description of the data and file structure

Data used for quantifying statistically significant differences in the molecular organization of EGFR are depicted in Figures 1 - 3. Statistical analyses were accomplished using generalized linear mixed effects models to estimate and compare differences between treatments/cell lines while accounting for the nested nature of the SR imaging datasets (with clustering metrics potentially being gathered from several ROIs within a cell, and potentially multiple cells being imaged per day). All underlying data, as well as code used for analysis of the data, can be found here. 

### Files and variables

#### File: 20251020_Fig1B-C_HeLa_dSTORM_ROIs.xlsx

**Description:** Values of Hopkins Statistics and relative number of clusters for EGFR in HeLa cells, as dep..., ,