GenBank accession numbers for ammonia oxidizer genes collected on the R/V Endeavor (SQO-Delta) in the San Francisco Bay Delta during September and October 2007.
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<p>Surface sediment samples were collected for potential nitrification rates, using sediment slurries with filtered site water. Ammonia oxidizer gene abundances (AOA and AOB <em>amoA</em>) were quantified using qPCR, and clone libraries for each gene were sequenced using Sanger sequencing.</p>
<p><strong>Related Manuscript: Damashel <em>et al., </em>2015</strong></p>
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本研究采用经采样位点过滤后的水体制备沉积物悬液，采集表层沉积物样本以测定潜在硝化速率。采用实时定量聚合酶链反应（quantitative Polymerase Chain Reaction，qPCR）对氨氧化古菌（AOA，Ammonia-oxidizing Archaea）与氨氧化细菌（AOB，Ammonia-oxidizing Bacteria）的amoA基因丰度进行定量，并针对两类基因分别构建克隆文库，采用桑格测序（Sanger sequencing）完成测序。
相关研究文献：Damashel等，2015
创建时间：
2021-12-05
