The impact of defective cholesterol metabolism on sex-specific hepatocarcinogenesis in hepatocyte specific deletion of Cyp51 in mice. The impact of defective cholesterol metabolism on sex-specific hepatocarcinogenesis in hepatocyte specific deletion of Cyp51 in mice

The potential role of defective cholesterol metabolism in HCC development and exposition the Cyp51 gene as a promising marker with tumor suppressor potential in sex-dimorphic chronic liver pathogenesis in mice. Hepatocyte-specific Cyp51 knock-out (KO) and wild type (WT) mice on a mixed background (129/Pas (10%) × C57BL/6J (90%)) of both sexes (F and M) were investigated at the age of 24 months. From each of KO mice, two samples (a tumor and a surrounding tissue) were considered. Overall design: Microarrays were hybridized with individual samples from livers of 14 mice (6 male, 8 female) at 24 months of age. 8 samples were hybridized from WT mice, i.e. 4 from male and 4 from female mice. 6 KO mice were investigated, i.e. 2 male and 4 female. From each of KO mice, two samples (a tumor and a surrounding tissue) were taken; altogether, 6 tumor and 6 corresponding surrounding-tissue samples were hybridized from KO mice.