Transcriptomic Analysis Reveals TME-Mediated Macrophage IFIT1 Upregulation and CX3CR1 Suppression Drive Osteosarcoma Progression

Osteosarcoma (OS) is one of the most common bone tumors which prognosis of patients is unsatisfactory. Due to the stagnancy in conventional treatments, researchers begin to find therapeutic targets from tumor microenvironment (TME) and tumor associated macrophages (TAM). We aim to investigate the influence of the effects of OS TME on gene expression of macrophages which may provide reference to OS treatment. RNA sequencing was performed on BMMs cultured with or without K7M2 CM for 48 hours to analyze gene expression changes. Transcriptome sequencing was performed on BMMs, BMMs after 48 h of K7M2 CM intervention, and BMMs after 48 hours of K7M2 CM + DTM intervention