Replication Data for: Notch2 is Involved in Regulating Apical Tissue Repair following Severe Intrusive Luxation

Study Purpose: The data generated in this study supports the investigation of apical repair dynamics in immature permanent teeth following severe intrusive luxation, and explores the regulatory role of the Notch signaling pathway in this repair process.
Methodology & Study Design: A Sprague-Dawley (SD) rat model of intrusive luxation was established using a modified tool. Data were collected through multiple analytical approaches: micro-computed tomography (Micro-CT) and hematoxylin-eosin (H&E) staining for evaluating tissue repair; differential gene expression analysis of stem cells from the apical papilla (SCAPs) using the public GEO dataset GSE149930; immunohistochemical detection of Notch2 receptor expression in the apical region of injured teeth (guided by bioinformatics screening); and pharmacological inhibition of the Notch signaling pathway with DAPT (γ-secretase inhibitor) to assess its impact on post-injury prognosis.
Data Collection Details: Histological data (from H&E staining) and imaging data (from Micro-CT) were collected to characterize pulp and apical tissue changes post-injury. Gene expression data of SCAPs were derived from the publicly accessible GEO dataset GSE149930, with additional immunohistochemical data capturing Notch2 expression levels in fibrotic apical regions. Outcome data related to tissue fibrosis and vascular proliferation were also collected following Notch signaling inhibition to quantify the pathway's regulatory effect.