Expression data from primary human bronchial epithelial basal cells from smokers non-COPD and COPD knocked down for SNAI2

Bronchial epithelial basal cells express high levels of SNAI2 and in order to identify the genes downstream of SNAI2, we performed a knockdown approach with shRNA specific of SNAI2 We used microarrays to compare the effect of SNAI2 knockdown versus a non-targetting sequences and identified genes that are up- or down-regulated downstream of SNAI2 Bronchial epithelial basal cells from smokers non-COPD and COPD, transduced by shRNA, either SNAI2 specific or non-targetting, were used for RNA extraction and hybridization on Affymetrix microarrays.