Gene expression data for genetic mode of control and inheritance identification for five strains of the two-spotted spider mite, Tetranychus urticae

We assessed expression using RNAseq for five strains of the two spotted spider mite, Tetranychus urticae (inbred strains SOL-BEi, ROS-ITi, JP-RRi, MR-VPi, and MAR-ABi). In the experimental design, four of the strains (ROS-ITi, JP-RRi, MR-VPi, and MAR-ABi) were crossed to the SOL-BEi strain, and RNA for RNAseq was collected from adult females of the parental strains and respective F1s. Using parental strain and F1 data, including allele-specific expression in F1s, we determined the mode of genetic control for gene expression variation in the respective contrasts. We also used the data to determine the mode of genetic inheritance. The analysis was based on RNAseq data and genomic DNAseq data released to NCBI under accessions PRJNA801103 and PRJNA799176 respectively. Datasets released here include (1) parent of origin RNA-seq read counts per gene for ASE analyses, (2) total RNAseq read counts per gene (no filters base on allelic states) used for assessing inheritance, and (3) variant data for pairwise comparisons of each of the four strains to SOL-BEi (the variant data was used in assessing ASE). The respective data is provided in three archives (a README file is provided in each archive).

本研究针对二斑叶螨（Tetranychus urticae）的5个近交品系（SOL-BEi、ROS-ITi、JP-RRi、MR-VPi及MAR-ABi），通过RNA测序（RNAseq）分析其基因表达水平。实验设计中，将其中4个品系（ROS-ITi、JP-RRi、MR-VPi与MAR-ABi）与SOL-BEi品系进行杂交，随后从亲本品系及对应F1代的成年雌螨中采集用于RNA测序的RNA样本。研究利用亲本品系与F1代的数据（包含F1代的等位基因特异性表达（allele-specific expression, ASE）数据），明确了各对比组中基因表达变异的遗传调控模式；同时借助上述数据解析了遗传模式。本分析基于分别上传至NCBI（美国国家生物技术信息中心）、登录号为PRJNA801103和PRJNA799176的RNA测序数据与基因组DNA测序（DNAseq）数据。本次发布的数据集包含三类：(1) 用于等位基因特异性表达分析的、按基因分类的亲本来源RNA测序读段计数数据；(2) 用于遗传模式评估的、按基因分类的总RNA测序读段计数数据（未基于等位基因状态进行过滤）；(3) 针对4个品系分别与SOL-BEi品系开展两两比对的变异数据（该变异数据用于等位基因特异性表达分析）。上述数据分别存储于三个压缩包中，每个压缩包均附带README说明文件。