Activation of NOTCH and BMP signaling pathways during acute hypoxic stress in cardiac mesenchymal cells

To analyze early transcriptional events in cardiac tissue after infarction and evaluate the genetic expression profile of post-infarction mesenchymal cells of the heart, we induced myocardial infarction in rats by ligation of the left coronary artery. 24 hours after surgery, the affected area was harvested for RNA isolation and cell culture. We then performed a gene expression profile analysis using data obtained from RNA sequencing of 3 different postinfarction tissues and cells. Healthy tissues and cells of the left ventricle of the heart of sham-operated rats were used as controls. Overall design: Comparative analysis of gene expression profiles of RNA sequencing data for postinfarction tissues and cardiac mesenchymal cells.