Joint-Structrual Biology Group (JSBG) BAG proposal for ID29

The aim of the JSBG BAG proposal for ID29 beamline is to develop new methods for time-resolved serial crystallography experiments at 4th generation source and to study time dependent structural dynamics of biological macromolecules that are central research topics at the ESRF-EMBL-GR Group. Method developments include the study of radiation damage with µs pulsed X-ray beam at high flux densities and with partially polychromatic X-rays (1% and 0.4%), the implementation and development of various sample delivery methods - mixing in capillary, microfluidics, tape-drive and fixed targets. This further includes development of GPU-based indexing algorithms, data visualization in ICAT, and development of new data collection strategies for pump-probe SSX experiments integrating a nanosecond laser. Biological systems include the study of photoactivable proteins, photoswitchable ligand driven structural dynamics, enzymology, and multi-temp based protein dynamics.