Supplementary Material for: Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp. H11

In recent years, large-scale outbreaks of the green alga Enteromorpha prolifera in China’s offshore waters have posed a serious threat. This study aimed to improve Enteromorpha polysaccharide (EP) enzymatic sugar production using the hydrolase system of Vibrio sp. H11, an EP-utilizing microbial strain. Strain H11 was found to contain 711 carbohydrate-related genes, and 259 genes belong to glycoside hydrolases that have the potential to hydrolyze EP. To maximize the capability of strain H11 to hydrolyze EP, both the culture medium and the composition were optimized. Response surface methodology analysis showed that maximal enzymatic production from strain H11 was 8.43 U/mL after 26-h incubation. When 50 g/L of EP were treated with crude H11 enzyme, the concentration of fermentation sugars increased by 36.12%. Under these conditions, the hydrolysates were capable of generating 3,217 mL/L of biogas and 6.74 g/L of biosolvents, with increases of 28.17 and 7.29%, respectively, compared to controls. The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.

近年来，中国近海水域中绿藻条浒苔（Enteromorpha prolifera）的大规模暴发已构成严重威胁。本研究旨在利用可利用浒苔多糖（EP）的微生物菌株弧菌（Vibrio sp.）H11的水解酶系统，提升浒苔多糖酶解产糖效率。经检测，菌株H11携带711个碳水化合物相关基因，其中259个属于糖苷水解酶（glycoside hydrolases）家族，具备水解EP的潜力。为最大化菌株H11的EP水解能力，本研究对培养基及其组成进行了优化。响应面法（Response surface methodology）分析结果显示，菌株H11在培养26小时后，酶活产量可达8.43 U/mL，为最大值。当以50 g/L的EP与菌株H11粗酶液进行反应时，发酵糖浓度提升了36.12%。在此优化条件下，该水解产物可产生3217 mL/L的沼气与6.74 g/L的生物溶剂，相较于对照组分别提升了28.17%与7.29%。将H11酶解系统与厌氧发酵技术联合应用，有望提升EP的综合利用价值。