Single cell RNA-seq of mouse cardiac cells: postnatal 3 weeks

We performed lineage tracing studies using various genetic models, and found that cardiac mesenchymal cells (MCs) contribute to CAs in postnatal development and adult homeostasis. We found that PDGFRb+ cells are heterogenous, and demonstrated that PDGFRb+ MCs, but not NG2+ coronary vascular mural cells, are the major source of intramyocardial adipocytes. We performed single cell RNA sequencing to compare the heterogeneity. Single cardiac cells were isolated from infant mouse at postnatal 3 weeks by retrograde Langendorff perfusion, Single cell library was generated using 10x Genomic Chromium system with the v3 single cell reagent kit. Sequencing of library was performed on Illumina NovoSeq 6000 platform.