Fif3 regulates the development of Penicillium oxalicum fungi and the expression of cellulolytic genes

The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT) and Fif3 deletion strain (DFif3) under conditions of 1% bran + 1% microcrystalline cellulose as carbon source. The results of correlation analysis between each sample showed that the gene expression level of DFif3 was significantly different from that of the WT strain. The deletion of DFif3 significantly down-regulates the expression levels of sporulation process genes. And the absence of DFif3 can broadly up-regulate the expression level of cellulase-encoding genes, indicating that DFif3 can cause a decrease in cellulase synthesis by affecting the expression of cellulase-encoding genes. Overall design: Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain, Fif3 deletion mutant strain in 18h in modified Mendal's medium with 1% bran + 1% microcrystalline cellulose as carbon sources. Three replicates were included for each samples.