Fish diversity in the Lake Kasumigaura watershed

Focusing on the Lake Kasumigaura watershed, we aimed to detect and assess trade-offs and synergies between multiple ecosystem services and biodiversity in order to receive ecosystem services sustainably. The Lake Kasumigaura watershed was divided into 50 sub-watersheds, and ecosystem services such as water quality and land use were examined in each watershed, as well as fish diversity as an indicator of biodiversity. Water quality and fish diversity were surveyed twice a year, in summer and winter, from 2016 to 2019. In this project, we investigated the fish diversity in each watershed regarding the use of environmental DNA extracted from river water. Metabarcoding was performed on amplicons using universal primers that amplify fish mitochondrial 12S rRNA (Miya et al. 2015).