Extended culture of 2D gastruloids to model human mesoderm development

Micropatterned human pluripotent stem cells (hPSCs) treated with BMP4, known as a 2D gastruloid, are among the most widely used stem cell models for human gastrulation. Due to its simplicity and reproducibility, this system is ideal for high throughput quantitative studies of tissue patterning and has led to many insights into the mechanisms of mammalian gastrulation. However, 2D gastruloids have only been studied up to 48h. Here we extended this system to 96h. Overall design: Micropatterned human pluripotent stem cells (hPSCs) were collected at 48, 72, 96 hours after treatment with BMP4 for single cell RNA sequencing with 10X Genomics Chromium Controller.