Defining the roles of NADPH oxidases in vascular remodelling and atherosclerosis: data

Researchers from Monash University (Grant Drummond, Christopher Sobey, Courtney Judkins, Henry Diep, Brad Broughton, Elizabeth Hooker, Alyson Miller, Stavros Selemidis) and the Howard Florey Institute (Greg Dusting) experimented on mice to test the hypothesis that upregulation of one enzyme (from a family of enzymes called NADPH oxidases) in the early stages of hypertension, is the initial trigger for many of the downregulation effects that ultimately lead to cardiovascular disease. Machine generated data, including flow cytometry, realtime PCR (Polymerase Chain Reaction) and FACS (Florescence Activated Cell Sorting) collected on blood plaque and blood pressure in mice was analysed to develop a better understanding of cause and prevention of heart attacks and strokes. The data revealed the underlying problem of atherosclerosis in blood vessels, the build up of fatty plaques which may close arteries or grow and rupture and the role of oxy-radicals and free-radicals in this build up of plaque. The enzyme NOX2 was identified as the probable cause.

莫纳什大学（Monash University）的格兰特·德拉蒙德（Grant Drummond）、克里斯托弗·索比（Christopher Sobey）、考特尼·贾德金斯（Courtney Judkins）、亨利·迪普（Henry Diep）、布拉德·布劳顿（Brad Broughton）、伊丽莎白·胡克（Elizabeth Hooker）、艾莉森·米勒（Alyson Miller）、斯塔夫罗斯·塞莱米迪斯（Stavros Selemidis），以及霍华德·弗洛里研究所（Howard Florey Institute）的格雷格·达斯廷（Greg Dusting），以小鼠为实验对象，验证了一项假说：高血压早期阶段，一种隶属于NADPH氧化酶（NADPH oxidases）家族的酶的上调，是最终引发心血管疾病的诸多下调效应的初始触发因素。研究团队对采集自小鼠血液斑块与血压相关的、经机器学习生成的实验数据进行了分析，这些数据涵盖流式细胞术（flow cytometry）、实时PCR（Polymerase Chain Reaction，聚合酶链反应）及荧光激活细胞分选术（FACS，Florescence Activated Cell Sorting）相关检测结果，旨在更深入地阐明心肌梗死与脑卒中的发病机制及预防策略。该数据集揭示了血管动脉粥样硬化的核心病理特征：脂肪斑块堆积可导致动脉闭塞、斑块增生甚至破裂，并明确了氧自由基与自由基在斑块堆积过程中的关键作用。研究最终确认NOX2酶为该病理过程的潜在致病诱因。