ZC3HC1 has functions distinct from TPR and is dispensable for TPR localization to the nuclear basket. ZC3HC1 has functions distinct from TPR and is dispensable for TPR localization to the nuclear basket

Background: The nuclear basket is a ‘fishtrap’-like structure on the nucleoplasmic face of the nuclear pore complex which has been implicated in diverse functions including RNA export, heterochromatin organisation, and mitosis. Recently, a novel component of the nuclear basket, ZC3HC1, has been described. The localisation of ZC3HC1 to nuclear pores has been reported to occur reciprocally with TPR, a major structural component of the nuclear basket. Methods and Results: Using immunofluorescence and RNA sequencing, here we show that in human fibroblasts, although ZC3HC1 localisation to nuclear pores is TPR-dependent, TPR localises to pores regardless of the presence of ZC3HC1. We demonstrate that knockdown of TPR and ZC3HC1 produce distinct transcriptional profiles. Conclusions: Our results suggest that there is little overlap in function between these two nuclear basket proteins Overall design: RNA-seq profiling of IMR90 cells treated with either siCTRL or siZC3HC1 for 3 days.