Additional file 1 of The hepato-ovarian axis: genetic evidence for a causal association between non-alcoholic fatty liver disease and polycystic ovary syndrome

Additional file 1: Table S1. Key characteristics of participating studies. Table S2. GWAS significant SNPs used as genetic instruments for fasting insulin and fasting glucose. Table S3. GWAS significant SNPs used as genetic instruments for serum SHBG levels and bioavailable testosterone levels in women. Table S4. Direct causal effects of NAFLD, fasting insulin, fasting glucose, serum SHBG levels, and serum bioavailable testosterone levels on PCOS risk via multivariable MR analysis. Table S5. Direct causal effects of NAFLD, fasting insulin, fasting glucose, and serum SHBG levels on serum bioavailable testosterone levels via multivariable MR analysis. Table S6. Direct causal effects of NAFLD, fasting insulin, and fasting glucose on serum SHBG levels via multivariable MR analysis. Table S7. Obesity-related genome-wide significant genetic variants. Table S8. Directional pleiotropy test using MR-Egger intercepts. Table S9. Horizontal pleiotropy test using MR-PRESSO. Table S10. Linkage disequilibrium score regression results on genetic correlations between NAFLD, fasting insulin, fasting glucose, SHBG, BT, and PCOS. Table S11. Indirect causal effects between NAFLD and PCOS via fasting insulin, serum SHBG levels, and serum bioavailable testosterone levels through step-wise MR analysis.

补充材料1：附表S1。纳入研究的核心特征。附表S2：用作空腹胰岛素与空腹血糖遗传工具变量的全基因组关联研究（Genome-Wide Association Study, GWAS）显著单核苷酸多态性（Single Nucleotide Polymorphisms, SNPs）。附表S3：用作女性血清性激素结合球蛋白（Sex Hormone-Binding Globulin, SHBG）水平与生物可利用睾酮水平遗传工具变量的GWAS显著SNPs。附表S4：通过多变量孟德尔随机化（Mendelian Randomization, MR）分析得出的非酒精性脂肪性肝病（Non-Alcoholic Fatty Liver Disease, NAFLD）、空腹胰岛素、空腹血糖、血清SHBG水平及血清生物可利用睾酮水平对多囊卵巢综合征（Polycystic Ovary Syndrome, PCOS）风险的直接因果效应。附表S5：通过多变量MR分析得出的NAFLD、空腹胰岛素、空腹血糖及血清SHBG水平对血清生物可利用睾酮水平的直接因果效应。附表S6：通过多变量MR分析得出的NAFLD、空腹胰岛素与空腹血糖对血清SHBG水平的直接因果效应。附表S7：与肥胖相关的全基因组显著遗传变异。附表S8：使用MR-Egger截距进行的方向性多效性检验。附表S9：使用MR-PRESSO（Mendelian Randomization Pleiotropy RESidual Sum and Outlier）进行的水平多效性检验。附表S10：针对NAFLD、空腹胰岛素、空腹血糖、SHBG、生物可利用睾酮（Bioavailable Testosterone, BT）与PCOS之间遗传相关性的连锁不平衡得分回归分析结果。附表S11：通过逐步MR分析得出的NAFLD经空腹胰岛素、血清SHBG水平及血清生物可利用睾酮水平对PCOS的间接因果效应。