Analysis of cellular transcripts targeted by ZAPL or ZAPS in HEK293T cells

To investigate the differential functions of two major ZAP variants, ZAPL and ZAPS, in the regulation of cellular RNA abundance, we established HEK293T cell lines in which either ZAPL or ZAPS has been knockout with CRISPR-mediated deletion. We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 genotypes (3 batches of WT; 2 and 1 batches of ZAPS-KO1 and 2, respectively; and 2 and 1 batches of ZAPL-KO1 and 2, respectively) and 2 treatments (medium-only and IFN-b treatment for 24h). Transcriptome profiles of HEK293T cell lines with either wild-type, ZAPL-KO or ZAPS-KO, using CRISPR/Cas9-mediated deletion