DNA Calorimetric Force Spectroscopy at Single Base Pair Resolution

The folder contains the raw data obtained from unzipping a 3653bp DNA hairpin at different temperatures with the optical-jump optical tweezers. Files are divided into two main folders, ‘highT’ and ‘lowT’, corresponding to the two setups possible with the force-jump optical tweezers. Inside these folders, data are grouped by acquisition date according to the format DDMMYY. Within each folder, the optical tweezers’ raw output is stored in the 'rawdata' folder. The raw data have to be pre-analyzed. Pre-analysis consists of selecting the data useful for our analysis (position, force, status, temperature). The raw data columns to be considered are the following: - Position, x = (A_dist-Y+B_dist-Y)/2 - Force, f = -Y_force - Status The status column stores info about the protocol and the temperature (unzipping: 1000, rezipping: 1001, and laser power: 02, 04, 06, 08, 10, etc.). The laser power corresponds to the second and third digits of the Status. Status values of less than four digits indicate that the unzipping/rezipping protocol was not started and have to be discarded. The temperature is measured as described in Sec. 1, Methods of the main text. To do this, refer to the file ‘stokes#.txt’ and extract the temperature corresponding to each laser power (stored in the ‘Status’ column as described above) using the Stokes law. To do this, use the ‘MotorVel_X’ column. Once these preliminary steps have been completed, one can proceed with the analysis described in the paper to measure the nearest-neighbor base-pairs thermodynamic parameters.