Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance

Pyramiding of alien-derived Wheat streak mosaic virus (WSMV) resistance and resistance enhancing genes in wheat is a cost-effective and environmentally safe strategy for disease control. PCR-based markers and cytogenetic analysis with genomic in situ hybridisation were applied to identify alien chromatin in four genetically diverse populations of wheat (Triticum aestivum) lines incorporating chromosome segments from Thinopyrum intermedium and Secale cereale (rye). Out of 20 experimental lines, 10 carried Th. intermedium chromatin as T4DL*4Ai#2S translocations, while, unexpectedly, 7 lines were positive for alien chromatin (Th. intermedium or rye) on chromosome 1B. The newly described rye 1RS chromatin, transmitted from early in the pedigree, was associated with enhanced WSMV resistance. Under field conditions, the 1RS chromatin alone showed some resistance, while together with the Th. intermedium 4Ai#2S offered superior resistance to that demonstrated by the known resistant cultivar Mac...

在小麦中聚合外源衍生的小麦条纹花叶病毒（Wheat streak mosaic virus, WSMV）抗性基因及抗性增强基因，是一种经济高效且环境友好的病害防控策略。本研究采用基于PCR的分子标记与基因组原位杂交（genomic in situ hybridisation, GISH）细胞遗传学分析方法，对4个遗传背景多样的普通小麦（Triticum aestivum）品系群体进行外源染色质鉴定，这些品系整合了中间偃麦草（Thinopyrum intermedium）与黑麦（Secale cereale, rye）的染色体片段。在20个试验品系中，10个携带中间偃麦草染色质，具体为T4DL·4Ai#2S易位；而出乎意料的是，有7个品系的1B染色体上携带外源染色质（中间偃麦草或黑麦）。本次新鉴定的源自系谱早期世代传递的黑麦1RS染色质，与增强的WSMV抗性显著相关。田间试验条件下，仅1RS染色质即可表现出一定抗性，当其与中间偃麦草4Ai#2S染色质联合应用时，其抗病效果优于已知抗性品种Mac...