Bathymetric adaptations of corals at Davies Reef, Great Barrier Reef: UV-B absorbing compounds

Corals were collected by scuba from depths between 1 and 45 m on the southeastern side of Davies Reef in October 1980 and February, July and December 1981 and the concentrations of mycosporine-like amino acids determined.Photosynthetic pigments and and mycosporine-like amino acids were extracted using 3 volumes of 20% tetrahydrofuran in methanol (v:v). Visible UV spectra were recorded and pigments were separated and quantified by high performance liquid chromatography. The UV-B band used in this study was 208-340 nm. To examine the relationship between the mycosporine-like amino acids of Acropora spp. and increasing depth.To determine whether Acropora spp. respond biochemically to the presence of UV-B light. Data analyses were part of a series of three papers addressing:1. Long-term growth responses (data not held by AIMS)2. Light saturation curves for photosynthesis and respiration (see separate metadata record)3. UV-B absorbing compoundsSee separate metadata records.

1980年10月及1981年2月、7月、12月，研究人员通过水肺潜水在戴维斯礁（Davies Reef）东南侧1至45米水深海域采集珊瑚样本，并测定了类菌胞素氨基酸（mycosporine-like amino acids）的浓度。采用3倍体积的甲醇中体积分数20%四氢呋喃溶液（体积比v:v）提取光合色素与类菌胞素氨基酸。记录可见紫外光谱，并通过高效液相色谱（High Performance Liquid Chromatography, HPLC）分离并定量色素。本研究使用的UV-B波段为208~340 nm。本研究旨在明确两个科学目标：一是探究鹿角珊瑚属（Acropora spp.）的类菌胞素氨基酸含量与水深增加之间的关联；二是验证鹿角珊瑚属是否会对UV-B辐射产生生化响应。本研究的数据分析属于三篇系列论文的研究内容，三篇论文的主题分别为：1. 长期生长响应（澳大利亚海洋科学研究所（Australian Institute of Marine Science, AIMS）未持有相关数据）；2. 光合作用与呼吸作用的光饱和曲线（详见独立元数据记录）；3. 紫外B吸收化合物。相关细节请查阅独立元数据记录。