Long Noncoding RNA AF131217.1 Regulated Coronary Slow Flow-Induced Inflammation Affecting Coronary Slow Flow via KLF4

Abstract Introduction: This study investigated the correlation between the levels of long noncoding ribonucleic acids (lncRNAs) AF131217.1 and coronary slow flow (CSF). Methods: A total of 22 patients in the high-sensitivity C-reactive protein (hsCRP) group diagnosed with CSF from January 2018 to December 2018 were enrolled in this study. Coronary flow velocity was determined using the thrombolysis in myocardial infarction frame count (TFC) method. Results: LncRNA AF131217.1 expression in the CSF model was activated. Mean TFC was positively correlated with lncRNA AF131217.1 levels and hsCRP levels. LncRNA AF131217.1 induced inflammation factor levels in the in vitro model. Micro ribonucleic acid (miR)-128-3p is a target spot of lncRNA AF131217.1 on the inflammation in vitro model via Kruppel-like factor (KLF) 4. MiR-128-3p reduced inflammation factor levels (tumor necrosis factor alpha, interleukin [IL]-6, IL-1β, and IL-18). Conclusion: Thus, lncRNA AF131217.1 promoted inflammation in the regulated CSF via KLF4 by miR-128-3p.

摘要 引言：本研究探讨了长链非编码核糖核酸（long noncoding ribonucleic acids，lncRNAs）AF131217.1的表达水平与冠状动脉慢血流（coronary slow flow，CSF）的相关性。方法：本研究纳入2018年1月至2018年12月期间确诊为CSF的高敏C反应蛋白（high-sensitivity C-reactive protein，hsCRP）组患者共22例。采用心肌梗死溶栓治疗临床试验帧数计数（thrombolysis in myocardial infarction frame count，TFC）法测定冠状动脉血流速度。结果：CSF模型中长链非编码核糖核酸AF131217.1的表达被激活。平均TFC值与长链非编码核糖核酸AF131217.1水平及hsCRP水平呈正相关。在体外炎症模型中，长链非编码核糖核酸AF131217.1可诱导炎症因子水平升高。微小核糖核酸（micro ribonucleic acid，miR）-128-3p是长链非编码核糖核酸AF131217.1通过Krüppel样因子（Krüppel-like factor，KLF）4在体外炎症模型中发挥调控作用的靶标。miR-128-3p可降低炎症因子水平（肿瘤坏死因子α、白细胞介素[IL]-6、IL-1β及IL-18）。结论：综上，长链非编码核糖核酸AF131217.1可通过miR-128-3p靶向调控KLF4，进而促进冠状动脉慢血流相关的炎症反应。