Dataset for: Screenhouse SPVD evaluations of 53 unique accessions of sweetpotato crop wild relative's species

A total of 53 accessions were evaluated for sweetpotato virus disease resistance. Virus carriers with SPVD were grafted on plants of parental accessions used for the crossing study. After four weeks of growth, plants were cut below the grafted node removing the virus carrier. Subsequently, plants were left to re-grow for another four weeks. On completion of the re-growth period plant samples were subjected to ELISA screening. Genotypes that were negative (no virus detected) for SPCSV or both virus were re-grafted with infected material and tested again for resistance. A negative control consisting of five I. trifida plants were grafted with healthy I. batatas (cv. Beauregard) scions which were tested negative. Scions of plants of those accessions that remained resistant were grafted on I. setosa as indicator plant to validate results.

本研究共对53份甘薯种质资源开展甘薯病毒病（sweetpotato virus disease, SPVD）抗性评估。将携带SPVD的病毒载体嫁接到杂交研究所用的亲本种质植株上，培养4周后，在嫁接节点下方截断植株并移除病毒载体。随后让植株继续再生4周，待再生周期结束后，采集植株样本进行酶联免疫吸附测定（Enzyme-Linked Immunosorbent Assay, ELISA）筛选。对甘薯褪绿矮化病毒（Sweet Potato Chlorotic Stunt Virus, SPCSV）或两种病毒均呈阴性（未检测到病毒）的基因型植株，再次使用带病毒的材料进行嫁接，并重新开展抗性检测。设置阴性对照：以5株三裂叶薯（I. trifida）为砧木，嫁接经检测呈阴性的健康甘薯（I. batatas）栽培品种‘博雷加德（Beauregard）’接穗。将仍表现出抗性的种质植株的接穗嫁接到刚毛薯（I. setosa）作为指示植株，以验证检测结果。