Additional file 2 of Identification of candidate genes responsible for chasmogamy in wheat

Additional file 2: Table S1. Fold changes of 594 down- and upregulated DEGs at the GAS and YAS of HCH (Pi-Piko, Po-Poezja) vs LCH (Eu-Euforia, Da-KWS Dacanto) cultivars identified in RNA-Seq analysis. Table S2. Expression levels of selected DEGs during Chinese Spring development ( http://wheatomics.sdau.edu.cn/expression/wheat.html ). Table S3. List of 1161 DEGs down- and upregulated at lodicules at the GAS and YAS of HCH (Pi-Piko, Po-Poezja) vs LCH (Eu-Euforia, Da-KWS Dacanto) cultivars identified in RDA-Seq analysis. Table S4. DEGs selected in RNA-seq and RDA-seq analyses, their sequences, location, promoter regions, and the best blast hits. Table S5. Regulatory elements identified in regions 2,000 bp upstream of selected DEGs. Table S6. DEGs identified in RNA-Seq analysis annotated in Merkator ( https://www.plabipd.de/portal/mercator4 ) database. Table S7. DEGs identified in RDA-Seq analysis annotated in Merkator ( https://www.plabipd.de/portal/mercator4 ) database. Table S8. Characteristics of 18 genes selected for qPCR analysis and PCR primers.

附加文件2：表S1。本表展示了RNA测序（RNA-Seq）分析中鉴定得到的594个在HCH（Pi-Piko、Po-Poezja）与LCH（Eu-Euforia、Da-KWS Dacanto）品种的绿色花药期（Green Anther Stage, GAS）和黄色花药期（Yellow Anther Stage, YAS）上下调的差异表达基因（Differentially Expressed Genes, DEGs）的表达倍数变化。
表S2。本表收录了中国春（Chinese Spring）发育过程中选定DEGs的表达水平，数据来源：http://wheatomics.sdau.edu.cn/expression/wheat.html。
表S3。该列表为在RDA测序（RDA-Seq）分析中鉴定得到的，HCH（Pi-Piko、Po-Poezja）与LCH（Eu-Euforia、Da-KWS Dacanto）品种的绿色花药期（Green Anther Stage, GAS）和黄色花药期（Yellow Anther Stage, YAS）的浆片（lodicules）中上下调的1161个DEGs。
表S4。收录了RNA-seq与RDA-seq分析中筛选得到的DEGs的序列、基因定位、启动子区域以及最优BLAST比对结果。
表S5。本表收录了选定DEGs上游2000 bp区域内鉴定得到的调控元件。
表S6。本表汇总了在Merkator数据库（https://www.plabipd.de/portal/mercator4）中完成功能注释的RNA-Seq分析鉴定得到的DEGs。
表S7。本表汇总了在Merkator数据库（https://www.plabipd.de/portal/mercator4）中完成功能注释的RDA-Seq分析鉴定得到的DEGs。
表S8。收录了用于实时定量聚合酶链式反应（quantitative PCR, qPCR）与聚合酶链式反应（Polymerase Chain Reaction, PCR）分析的18个选定基因的特征信息及引物序列。