Single cell RNAseq of mouse CD3+ Adipose Tissue T cells

Despite studies implicating adipose tissue T cells (ATT) in the initiation and persistence of adipose tissue inflammation, fundamental gaps in knowledge regarding ATT function impedes progress towards understanding how obesity influences adaptive immunity. We hypothesized ATT activation and function would have tissue-resident specific properties and that obesity would potentiate their inflammatory properties. We assessed ATT activation and inflammatory potential within mouse stromal vascular fraction (SVF).To assess intracellular signaling mechanisms responsible for ATT inflammation impairments, single-cell RNA sequencing of ATTs from epididymal adipose tissue was performed from male chow and high fat diet (60% for 12 weeks) fed mice. FACS sorted CD3+ cells from epididymal adipose tissue from male lean (n=2) and HFD fed (n=1) C57Bl/6 mice were analyzed by single cell RNA-seq (10X genomics)尽管已有研究表明脂肪组织T细胞（adipose tissue T cells，后文简称ATT）参与脂肪组织炎症的启动与维持，但目前关于ATT功能的基础性认知空白，仍阻碍了学界对肥胖如何影响适应性免疫的研究进展。我们提出假说：ATT的激活与功能具备组织驻留特异性特征，且肥胖会强化其促炎特性。我们在小鼠基质血管组分（stromal vascular fraction，后文简称SVF）中评估了ATT的激活状态与促炎潜能。为解析介导ATT炎症功能异常的胞内信号机制，我们对喂食普通饲料与高脂饮食（60%脂肪供能，持续12周，后文简称HFD）的雄性小鼠的附睾脂肪组织中的ATT开展了单细胞RNA测序。我们通过单细胞RNA测序（10X Genomics）分析了经荧光激活细胞分选术（FACS）分选出的CD3阳性T细胞，这些细胞采自2只雄性瘦型C57BL/6小鼠（n=2）与1只高脂饮食喂养的雄性C57BL/6小鼠（n=1）。