RNAseq profiling of miR-132/212-deficient CD4+ T cells activated in vitro and in vivo.

Transcriptomic profiling of miR-132/212-deficient and WT CD4 T cells isolated from spleens of L donovani infected mice (d28) to determine the effects of miR-132/212 on CD4 T cell activation in vivo. This was combined by transcriptomic analysis of early stage in vitro activated WT and miR-132/212-deficient CD4 T cells to identify direct miR-132/212 targets in CD4 T cells. Examination of expression profiles of splenic CD4+ T cells from L. donovani-infected WT (samples 1-4) and miR-132/212-/- mice (samples 5-9) using RNASeq. This was followed by similar RNASeq in naïve CD4+ T-cells in WT and miR-132/212 -/- mice prior to and following 18h of in vitro TCR stimulation under Th1 conditions (samples 10-25).