Gene expression analysis of frozen skin from B6 mouse models of Cutaneous Lupus Erythematosus and Primary Cicatricial Alopecia. Gene expression analysis of frozen skin from B6 mouse models of Cutaneous Lupus Erythematosus and Primary Cicatricial Alopecia

OT1 CD8 T cells or OT2 Th2 T cells were injected into rtTA+K5TGO+TLR9KO recipients or littermate controls. A portion of the skin was frozen and saved for RNA analysis at the first presentation of disease (approximately 3-4 weeks). OT2 recipient skin was also harvested at flare (approximately 10-12 weeks). All extracted RNA samples were analyzed using NanoString mouse Cancer Immunology panel (C3400). Overall design: OT1 CD8 T cells or OT2 Th2 T cells were injected into rtTA+K5TGO+TLR9KO recipients or littermate controls. A portion of the skin was frozen and saved for RNA analysis at the first presentation of disease (approximately 3-4 weeks). OT2 recipient skin was also harvested at flare (approximately 10-12 weeks). All extracted RNA samples were analyzed using NanoString mouse Cancer Immunology panel (C3400).