Role of B7H4 and Fas in regulation of trophoblast cells and development of pre-eclampsia: a cross-sectional study

This study aimed to investigate the role of B7H4 and Fas in pre-eclampsia (PE) occurrence and development and reveal its potential mechanisms. Thirty healthy individuals and 60 patients with PE were enrolled in the study. In addition, the clinical characteristics of all participants were collected, including age, gestational weeks at delivery, gestational time, number of births, systolic blood pressure, diastolic blood pressure and foetal weight. The chi-square test was used to evaluate differences in clinical characteristics between the high- and low-expression groups. The expression levels of B7H4 and Fas were analysed using western blotting and real-time quantitative polymerase chain reaction (RT-qPCR). The upstream regulators of B7H4 in trophoblasts were predicted and estimated using a luciferase reporter assay. The proliferation and motility of trophoblasts were assessed using CCK8 and transwell assays, respectively. B7H4 and Fas were upregulated in PE (p p p p miR-4319 mediates the progression of trophoblast progression by negatively regulating the expression of B7H4 and Fas. Therefore, B7H4 and Fas may serve as potential biomarkers for the prediction and treatment of PE. This study aimed to evaluate the significance of B7H4 and Fas in the occurrence and development of pre-eclampsia (PE) and to reveal its potential mechanism. We found that the upregulated B7H4 and Fas served as diagnostic biomarkers for PE, and their combination showed higher diagnostic efficacy, sensitivity and specificity. miR-4319 negatively regulates B7H4 and Fas in trophoblasts and therefore mediates cell proliferation, migration and invasion.