Raw qPCR data for RNA-seq validation

Data file consists of two excel sheets. The first sheet labelled 'ct' contains all raw Ct values that informed the qPCR analysis. Column headings are gene IDs for that particular gene. Information on qPCR primers and efficiency can be found in the Supplementary information. 'Treatment' = phenotype tested. 'Biorep' = biological replicate. 'Techrep' = technical replicate. The second sheet ('validation') contains the fold-change comparisons that informed the RNA-seq validation analysis. Validations were performed for each software package - edgeR and DEseq2. Comparisons were made only in instances when the gene was significantly differentially expressed in the RNA-seq.本数据集文件包含两张Excel工作表。第一张名为"ct"的工作表收录了所有用于实时荧光定量PCR（quantitative real-time polymerase chain reaction, qPCR）分析的原始Ct值，其列标题为对应基因的基因标识符（gene ID）。实时荧光定量PCR引物及扩增效率的相关信息可参见补充材料。其中，"Treatment"代表所检测的表型，"Biorep"代表生物学重复（biological replicate），"Techrep"代表技术重复（technical replicate）。第二张名为"validation"的工作表收录了用于RNA测序（RNA-seq）验证分析的倍数变化比较结果。验证分析分别基于edgeR和DEseq2两款软件开展，且仅当基因在RNA-seq分析中呈显著差异表达时，才会进行此类比较。