Elongated Dmc1 structures and higher order clustered Rad51 structures accumulate in strand exchange mutants at late times.

(A-D) 8 hr mnd1 nucleus stained for Dmc1. (A) dSTORM and (B) widefield micrographs each with 1 μm scale bars. (C) Magnified region from (A) indicated by arrowhead, highlighting elongated Dmc1 structure. Scale bar is 100 nm wide. (D) Surface plot of (C). (E-H) 3.5 hr dmc1 nucleus stained for Rad51, highlighting a common pair (low order cluster) of sr foci. (I-L) 8 hr mnd1 nucleus and (M-P) 8 hr dmc1 nucleus stained for Rad51, highlighting higher order clusters of Rad51 sr foci. (Q-T) dSTORM micrographs of elongated Rad51 structures observed in spread nuclei of mitotic srs2 mutants overexpressing Rad51. Scale bar is 1 μm.