Variations in the composition and frequency of celiac disease epitopes and immunogenicity among synthetic wheat lines

We performed RNA amplicon sequencing of the most toxic region of alpha-gliadins to analyze the content and composition of CD-related alpha-gliadin epitopes across eight synthetic wheat lines developed from crosses between durum wheat and different Aegilops species containing the D-genome (Ae. tauschii, Ae. crassa, and Ae. ventricosa). This project contains row sequence reads of eight synthetic wheats. The reads belonging to each genotype could be identified via the ID oligo sequence of the forward primer. Please see the related paper to inform how to separate row sequence reads for each genotype.